用于细胞生物和组织工程学研究的原子力显微镜

• 治疗后的活细胞的动态成像
• 癌细胞中的刚度和粘弹性变化
• 细胞基质对细胞分化的影响
•  细胞对机械刺激的反应

"Peptide density targets and impedes triple negative breast cancer metastasis," D. Liu, P. Guo, C. McCarthy, B. Wang, Y. Tao, and D. Auguste, Nat. Commun. 9, 2612 (2018). https://doi.org/10.1038/s41467-018-05035-5

"Direct micropatterning of extracellular matrix proteins on functionalized polyacrylamide hydrogels shows geometric regulation of cell–cell junctions," B. Sarker, C. Walter, and A. Pathak, ACS Biomater. Sci. Eng. 4, 2340 (2018). https://doi.org/10.1021/acsbiomaterials.8b00331

"Measuring nanoscale viscoelastic parameters of cells directly from force-displacement curves," Y. M. Efremov, W.-H. Wang, S. D. Hardy, R. L. Geahlen, and A. Raman, Sci. Rep. 7, 1541 (2017). https://doi.org/10.1038/s41598-017-01784-3

"3D cell bioprinting of self-assembling peptide-based hydrogels," B. Raphael, T. Khalil, V. L. Workman, A. Smith, C. P. Brown, C. Streuli, A. Saiani, and M. Domingos, Mater. Lett. 190, 103 (2017). https://doi.org/10.1016/j.matlet.2016.12.127

"T cell activation requires force generation," K. H. Hu and M. J. Butte, J. Cell Biol. 213, 535 (2016). https://doi.org/10.1083/jcb.201511053

"Mechanical heterogeneities in the subendothelial matrix develop with age and decrease with exercise," J. C. Kohn, A. Chen, S. Cheng, D. R. Kowal, M. R. King, and C. A. Reinhart-King, J. Biomech. 49, 1447 (2016). https://doi.org/10.1016/j.jbiomech.2016.03.016

"Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration," I. Acerbi, L. Cassereau, I. Dean, Q. Shi, A. Au, C. Park, Y. Y. Chen, J. Liphardt, E. S. Hwang, and V. M. Weaver, Integr. Biol. 7, 1120 (2015). https://doi.org/10.1039/c5ib00040h

"Nanomechanics of cells and biomaterials studied by atomic force microscopy," J. I. Kilpatrick, I. Revenko, and B. J. Rodriguez, Adv. Healthcare Mater. 4, 2456 (2015). https://doi.org/10.1002/adhm.201500229

"Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression," J. K. Mouw, Y. Yui, L. Damiano, R. O. Bainer, J. N. Lakins, I. Acerbi, G. Ou, A. C. Wijekoon, K. R. Levental, P. M. Gilbert, E. S. Hwang, Y.-Y. Chen, and V. M. Weaver, Nat. Med. 20, 360 (2014). https://doi.org/10.1038/nm.3497

"Atomic force microscopy-based microrheology reveals significant differences in the viscoelastic response between malign and benign cell lines," J. Rother, H. Nöding, I. Mey, and A. Janshoff, Open Biol. 4, 140046 (2014). https://doi.org/10.1098/rsob.140046

"A physical sciences network characterization of non-tumorigenic and metastatic cells," The Physical Sciences – Oncology Centers Network (D. B. Agus et al.), Sci. Rep. 3, 1449 (2013). https://doi.org/10.1038/srep01449

"Comparison of the viscoelastic properties of cells from different kidney cancer phenotypes measured with atomic force microscopy," L. M. Rebelo, J. S. de Sousa, J. Mendes Filho, and M. Radmacher, 24, 055102 (2013). https://doi.org/10.1088/0957-4484/24/5/055102

"Elasticity maps of living neurons measured by combined fluorescence and atomic force microscopy," E. Spedden, J. D. White, E. N. Naumova, D. L. Kaplan, and C. Staii, Biophys. J. 103, 868 (2012). https://doi.org/10.1016/j.bpj.2012.08.005

"Cell stiffness is a biomarker of the metastatic potential of ovarian cancer cells," W. Xu, R. Mezencev, B. Kim, L. Wang, J. McDonald, and T. Sulchek, PLoS One 7, e46609 (2012). https://doi.org/10.1371/journal.pone.0046609

"In situ force mapping of mammary gland transformation," J. I. Lopez, I. Kang, W.-K. You, D. M. McDonald, and V. M. Weaver, Integr. Biol. 3, 910 (2011). https://doi.org/10.1039/c1ib00043h

"Mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy," A. Raman, S. Trigueros, A. Cartagena, A. P. Z. Stevenson, M. Susilo, E. Nauman, and S. A. Contera, Nat. Nanotechnol. 6, 809 (2011). https://doi.org/10.1038/nnano.2011.186

"Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes," H. Jin, D. A. Heller, M. Kalbacova, J.-H. Kim, J. Zhang, A. A. Boghossian, N. Maheshri, and M. S. Strano, Nat. Nanotechnol. 5, 302 (2010). https://doi.org/10.1038/nnano.2010.24

"Nanotopography-induced changes in focal adhesions, cytoskeletal organization, and mechanical properties of human mesenchymal stem cells," E. K. Yim, E. M. Darling, K. Kulangara, F. Guilak, and K. W. Leong, Biomaterials 31, 1299 (2010). https://doi.org10.1016/j.biomaterials.2009.10.037

"Impact of actin rearrangement and degranulation on the membrane structure of primary mast cells: A combined atomic force and laser scanning confocal microscopy investigation," Z. Deng, T. Zink, H. Chen, D. Walters, F. Liu, and G. Liu, Biophys. J. 96, 1629 (2009). https://doi.org/10.1016/j.bpj.2008.11.015

"Control of myocyte remodeling in vitro with engineered substrates," N. A. Geisse, S. P. Sheehy, and K. K. Parker, In Vitro Cell. Dev. Biol.–Animal 45, 343 (2009). https://doi.org/10.1007/s11626-009-9182-9

"Intermediate filament-like proteins in bacteria and a cytoskeletal function in Streptomyces," S. Bagchi, H. Tomenius, L. M. Belova, and N. Ausmees, Mol. Microbiol. 70, 1037 (2008). https://doi.org/10.1111/j.1365-2958.2008.06473.x

"A cell nanoinjector based on carbon nanotubes," X. Chen, A. Kis, A. Zettl, and C. R. Bertozzi, Proc. Natl. Acad. Sci. U. S. A. 104, 8218 (2007). https://doi.org/10.1073/.0700567104

"Matrix elasticity directs stem cell lineage specification," A. J. Engler, S. Sen, H. L. Sweeney, and D. E. Discher, Cell 126, 677 (2006). https://doi.org/10.1016/j.cell.2006.06.044

"Indentation and adhesive probing of a cell membrane with : Theoretical model and experiments," S. Sen, S. Subramanian, and D. E. Discher, Biophys. J. 89, 3203 (2005). https://doi.org/10.1529/biophysj.105.063826

"Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments," A. J. Engler, M. A. Griffin, S. Sen, C. G. Bönnemann, H. L. Sweeney, and D. E. Discher, J. Cell Biol. 166, 877 (2004). https://doi.org/10.1083/jcb.200405004