This force-distance curve is an example of force spectroscopy measurements. Here, a protein called titin was unfolded under the force of the atomic force microscope. Each peak corresponds to the unfolding of one domain in the protein.

原子力显微镜是一种功能强大的仪器,能够测量各种材料的机械性能,从软生物材料(分子、细胞、组织等),到聚合物和较坚固的无机材料等。根据悬臂的弹簧常数,当任意一个分子被展开(分子内),或两个分子被分开(分子间),即可进行 皮牛(pN)级别的力学测量。

  • 单分子力谱
  • 在pN力分辨率下分开分子
  • 在受控的触发因素下(力或偏转),对材料施加推力,以测量杨氏模量
  • 力夹紧
  • 力增加
  • 在相关的溶液和温度下操作
  • 化学力显微镜
  • 胶体探针
  • 解开/展开模块化蛋白质,测量/观察能量图景。
  • 释放/融化DNA分子(B-S转换)
  • 聚合物的构象变化
  • 测量分子间的作用力:配合基-受体对、抗体-抗原结合、蛋白质-矿物。
  • 在功能组和表面之间测量特异性
  • 测量附着力
  • 对细胞(固定细胞和活细胞)、聚合物和凝胶进行机械测量

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