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The Closed Fluid Cell (CFC) for MFP-3D AFMs is designed to hold gases or liquids either statically or with perfusion through configurable inlet/outlet ports. The unique design enables the cell, sample, and cantilever holder to be fully assembled and sealed in a controlled environment (e.g. glovebox or hood) and then transferred to the AFM for use. Two related versions are available: 1) the “Fluid Cell Lite” that has no inlet/outlet ports and minimizes evaporation but is not completely sealed, and 2) the “Electrical Closed Cell,” which enables electrical connections to the sample.